Buccal DNA samples for DNA typing: new collection and processing methods
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چکیده
منابع مشابه
Buccal DNA samples for DNA typing: new collection and processing methods.
With the recent expansion of DNA database laws in many states, there is a critical need for the rapid and simple collection of DNA samples and streamlined processing for downstream applications. The Buccal DNA Collector was developed to address the need for a reliable, practical alternative to blood collection that is compatible with high-throughput operations. The collection area consists of f...
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It is considered undesirable to take blood from an infant as a means of obtaining DNA for research purposes. Sufficient DNA for direct gene analysis can be obtained from adult buccal epithelial cells, 1 but there is no evidence that neonatal buccal epithelial cells can similarly be used. We have carried out a preliminary study, approved by the Riverside Research Ethics Committee, to determine t...
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Collection and analysis of DNA, most commonly from blood or buccal cells, is becoming more common in epidemiologic studies. Buccal samples, which are painless to take and relatively easily collected, are often the preferred source. There are several buccal cell collection methods: swabs, brushes, mouthwash, and treated cards, such as FTA or IsoCode cards. Few studies have systematically compare...
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There is considerable interest in noninvasive and cost-effective methods for obtaining DNA in large-scale studies. In this randomized crossover study of 22 participants, we compared the DNA yield, quality, and associated costs of buccal cell DNA collected using cytobrushes (three brushes per collection) and swish (i.e., mouthwash) in self-administered procedures. There was a nonstatistically si...
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The term HLA refers to the Human Leukocyte Antigen System, which is controlled by genes on the short arm of chromosome six. The HLA loci are part of the genetic region known as the major histocompatibility complex (MHC). The MHC has genes (including HLA) that form part of the normal function of the immune response. The essential role of the HLA antigens lies in the control of self-recognition a...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2005
ISSN: 0736-6205,1940-9818
DOI: 10.2144/05392pf01